The influence of Mexidol on the expression of the NRF2 transcription factor in the cortex of the large hemispheres of the brain with experimental ischemia

Authors:
YAKUSHEVA E.N., MYLNIKOV P.Yu., CHERNYKH I.V., SHCHULKIN A.V.

FGBOU in the Ryazan State Medical University named after Acad. I.P. Pavlova "of the Ministry of Health of Russia, Ryazan, Russia

Place of publication:
JOURNAL OF NEUROLOGY AND PSYCHIATRY, 5, 2018

Abstract:
Objective. To study the effect of the original domestic drug Mexidol on the expression of the transcription factor Nrf2 in the nuclei of frontal cortex cells in the brain after unilateral occlusion of the common carotid artery. Material and Methods. The study was performed on 64 sexually mature male Wistar rats. Nrf2 expression was determined by immunohistochemistry. Results and Conclusion. A single intraperitoneal administration of Mexidol at a dose of 120 mg per 1 kg of body weight and a course of oral administration at a dose of 100 mg per 1 kg of body weight 3 times a day for 14 days did not affect the expression of the Nrf2 factor. Unilateral occlusion of the common carotid artery increased Nrf2 expression 4 hours and on the 5th day after the occlusion modeling. Oral administration of 100 mg of Mexidol/kg body weight three times daily for 14 days before and after simulated ischemia increased Nrf2 expression after 4 hours and on day 12 compared to normal values, as well as after 4 hours and on day 12 compared to occlusion control values. Thus, it was established that Mexidol increases Nrf2 expression in the frontal cortex of rats not under normal conditions, but after unilateral occlusion of the common carotid artery. Key words: Mexidol, Nrf2, ethylmethylhydroxypyridine succinate, ischemia, common carotid artery occlusion.